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Botany JoVE (Open Access) Citable · DOI

Method of Direct Segmental Intra-hepatic Delivery Using a Rat Liver Hilar Clamp Model

DOI: 10.3791/54729-v
What you'll learn
  • ✓ Perform rat liver hilar clamping and segmental ischemia induction
  • ✓ Cannulate portal vein branches for direct hepatic drug delivery
  • ✓ Monitor ischemia-reperfusion injury and collect hepatic samples during reperfusion
Protocol

A unique rat liver hilar clamp model was developed for studying the impact of pharmacologic molecules in ameliorating ischemia-reperfusion injury. This model includes direct cannulation of the portal supply to the ischemic liver segment via a branch of the portal vein, allowing for direct hepatic delivery.

Difficulty
advanced
Total time
~3–4 hours per rat (surgical preparation, ischemia window, reperfusion, sampling)
Model organism
Rat (species not specified in abstract; likely Sprague-Dawley or Lewis)
Biosafety
BSL-1

Steps

1
Position rat and expose hepatic hilum

Surgically access the rat liver and identify the hilar structures to enable vascular clamping of the hepatic blood supply to a specific liver segment.

▶ 00:57
2
Apply hilar clamp and establish portal cannulation

Place a vascular clamp across the hepatic hilum to induce segmental ischemia, then directly cannulate a portal vein branch to enable segmental intrahepatic drug delivery.

▶ 05:44
3
Monitor ischemia and initiate reperfusion sampling

Monitor the ischemic period, remove the hilar clamp to allow reperfusion, and collect hepatic tissue or blood samples at defined reperfusion timepoints.

▶ 07:19
4
Assess direct intrahepatic delivery efficacy outcomes

Analyze collected samples to evaluate the protective effects of directly delivered pharmacologic molecules (e.g., PEG-SOD) on ischemia-reperfusion injury markers.

▶ 08:43
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