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Botany JoVE (Open Access) Citable · DOI

Microbead Implantation in the Zebrafish Embryo

DOI: 10.3791/52943-v
What you'll learn
  • ✓ Prepare zebrafish embryos and microbeads for implantation procedures
  • ✓ Execute precise microbead implantation into developing embryo tissue
  • ✓ Assess developmental outcomes following bead manipulation intervention
Protocol

The zebrafish is an excellent model system for genetic and developmental studies. Bead implantation is a valuable tissue manipulation technique that can be used to interrogate developmental mechanisms by introducing alterations in local cellular environments. This protocol describes how to perform microbead implantation in the zebrafish embryo.

Difficulty
intermediate
Total time
~2–3 hours per experimental batch (embryo collection + implantation + initial observation)
Model organism
Zebrafish (Danio rerio) embryo
Biosafety
BSL-1

Steps

1
Prepare microbead implantation tray setup

Assemble and organize the tray system required for staging and manipulating zebrafish embryos during microbead implantation. This includes preparing the working surface and bead placement materials.

▶ 01:09
2
Collect and stage zebrafish embryos

Obtain zebrafish embryos at the appropriate developmental stage for implantation. Prepare embryos for the manipulation procedure by positioning them in the implantation tray.

▶ 02:15
3
Perform microbead implantation in embryo

Execute the precise implantation of microbeads into the target embryonic tissue using micromanipulation techniques to alter local cellular environment and interrogate developmental mechanisms.

▶ 03:02
4
Assess post-implantation zebrafish development

Monitor and evaluate gross developmental outcomes in implanted embryos to confirm that bead insertion does not cause overt developmental perturbations and to observe experimental effects.

▶ 04:36
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