A highly reproducible model for myocardial infarction in mice with minimal invasive manipulations is described. The model can be easily performed, resulting in a high reproducibility and survival rate. Thus, the described model will reduce the number of required animals as requested by the 3R principle (Replacement, Refinement and Reduction).
Total time
~45–60 min per mouse (including anesthesia, surgery, recovery, and initial analysis)
Steps
1
Intubate and anesthetize mouse
Prepare and intubate the mouse with appropriate anesthetic agents to maintain general anesthesia throughout the surgical procedure.
▶ 01:19
2
Induce myocardial infarction surgically
Access the left anterior descending coronary artery and perform ligation or occlusion to create the infarction model.
▶ 02:34
3
Close incision and recover mouse
Suture the surgical site and carefully manage post-operative recovery under monitored conditions.
▶ 04:13
4
Assess myocardial infarction extent
Perform imaging and histological analysis to quantify infarct size and cardiac function using standard analytical methods.
▶ 04:57
5
Document representative infarction results
Collect and present representative data demonstrating reproducibility of the infarction model across multiple animals.
▶ 06:50