The surgical procedure for induction of unilateral hindlimb ischemia is demonstrated, with confirmation of ischemia by laser Doppler perfusion imaging.
Difficulty
advanced
Total time
~90–120 min/mouse (surgical procedure plus imaging)
Model organism
Mouse (strain not specified)
Biosafety
BSL-1
Steps
1
Understand hindlimb ischemia model rationale
Review the scientific background and clinical relevance of unilateral hindlimb ischemia as a model system for studying vascular disease and tissue perfusion.
▶ 01:23
2
Induce unilateral hindlimb ischemia surgically
Perform the surgical procedure to create unilateral hindlimb ischemia in anesthetized mice, including vessel ligation or occlusion techniques.
▶ 02:15
3
Measure blood perfusion by laser Doppler imaging
Use laser Doppler perfusion imaging to non-invasively confirm ischemia induction and quantify changes in blood flow to the hindlimb.
▶ 07:11
4
Analyze and interpret perfusion imaging outcomes
Review representative results and outcome measurements demonstrating successful ischemia induction and perfusion deficit visualization.
▶ 10:21
💬 Comments coming soon
New protocols and pitfalls, in your inbox
A short email when we add notable lab videos and failure cases. No spam, unsubscribe anytime.