This protocol describes the porcine myocardial infarction (MI) model using a 90 min closed-chest coronary balloon occlusion of the left anterior descending artery (LAD), followed by reperfusion. Furthermore, the protocol for several outcome parameters, such as cardiac function, hemodynamics, microvascular resistance, and infarct size, are also presented.
Total time
~4-6 hours per pig (surgical preparation through functional assessment)
Model organism
Pig (Sus scrofa)
Steps
1
Perform transthoracic echocardiography baseline assessment
Obtain baseline cardiac imaging and functional parameters using ultrasound before intervention.
▶ 02:02
2
Prepare surgical site and establish vascular access
Perform sterile preparation, cannulate femoral artery and vein for catheter-based intervention and monitoring.
▶ 02:56
3
Conduct invasive pressure-volume loop analysis
Insert conductance catheter into left ventricle to measure real-time pressure and volume dynamics.
▶ 04:23
4
Measure intracoronary pressure and flow parameters
Deploy pressure-temperature sensor wire in left anterior descending artery to quantify coronary hemodynamics.
▶ 05:56
5
Induce myocardial infarction via LAD occlusion
Position balloon catheter at LAD and perform 90 minutes of closed-chest coronary occlusion followed by reperfusion.
▶ 07:00
6
Assess infarct size and cardiac remodeling outcomes
Quantify infarct size, measure changes in cardiac function and structural remodeling post-reperfusion using imaging and hemodynamic data.
▶ 08:58