Home › Biochemistry › NanoDrop Microvolume Quantitation of Nucleic Acids
Biochemistry JoVE (Open Access) Citable · DOI

NanoDrop Microvolume Quantitation of Nucleic Acids

DOI: 10.3791/2565-v
What you'll learn
  • ✓ Operate NanoDrop 2000c for nucleic acid quantitation
  • ✓ Measure high-sensitivity samples with NanoDrop 3300
  • ✓ Compare microvolume and traditional quantitation methods
  • ✓ Apply proper sample retention and measurement techniques
Protocol

Biopharma Insights The use of NanoDrop microvolume systems as practical and efficient alternatives to traditional nucleic acid quantitation methodology is described through the demonstration of two microvolume nucleic acid quantitation protocols.

Difficulty
beginner
Total time
~30 min per sample set

Steps

1
Operate NanoDrop sample retention system

Learn how the sample retention system works to hold microvolume samples without cuvettes.

▶ 00:05
2
Quantify nucleic acids using NanoDrop 2000c

Demonstrate microvolume spectrophotometric quantitation of nucleic acid samples, including blanking and measurement.

▶ 01:25
3
Perform high-sensitivity quantitation with NanoDrop 3300

Use the fluorospectrometer with fluorescent dyes for sensitive quantitation of low-concentration nucleic acids.

▶ 04:11
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