Home › Cell Biology › Neural Tube Closure in Mouse Whole Embryo Culture
Cell Biology JoVE (Open Access) Citable · DOI

Neural Tube Closure in Mouse Whole Embryo Culture

DOI: 10.3791/3132-v
What you'll learn
  • ✓ Prepare culture medium and isolate embryos
  • ✓ Set up whole embryo culture system
  • ✓ Evaluate neurulation after culture
  • ✓ Assess representative embryo cultures
Protocol

Biopharma Insights A method allowing for direct pharmacological manipulation of mouse embryos during neurulation that bypasses maternal metabolism is described. The technique can be adapted to study different aspects of neurulation by varying the time point and pharmacological agent.

Difficulty
advanced
Total time
~3-4 hours per experiment
Model organism
Mouse
Biosafety
BSL-1

Steps

1
Prepare medium and isolate embryos

Prepare culture medium and isolate uterus to remove embryos at the desired stage.

▶ 01:47
2
Set up whole embryo culture system

Assemble the culture apparatus and place embryos in culture conditions.

▶ 03:59
3
Evaluate development after whole embryo culture

Assess embryo development, including neural tube closure and general morphology.

▶ 05:20
4
Review representative embryo cultures

Examine examples of cultured embryos to ensure proper technique and expected outcomes.

▶ 06:10
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