Home › Botany › Normothermic Ex Vivo Kidney Perfusion for the Preservation of Kidney Grafts prior to Transplantation
Botany JoVE (Open Access) Citable · DOI

Normothermic Ex Vivo Kidney Perfusion for the Preservation of Kidney Grafts prior to Transplantation

DOI: 10.3791/52909-v
What you'll learn
  • ✓ Set up and prime a normothermic ex vivo kidney perfusion circuit
  • ✓ Perform organ retrieval and graft preparation for NEVKP
  • ✓ Evaluate kidney graft viability using perfusion parameters
  • ✓ Apply NEVKP preservation technique prior to transplantation
Protocol

The severe organ shortage has resulted in increased use of marginal kidney grafts for transplantation. This has triggered interest in alternative storage methods, since marginal grafts especially tolerate cold storage poorly. The technique of normothermic ex vivo kidney perfusion (NEVKP) represents a novel preservation method for kidney grafts prior to transplantation.

Difficulty
advanced
Total time
~4–6 hours (organ retrieval through perfusion setup and monitoring)
Biosafety
BSL-1

Steps

1
Retrieve donor kidney graft

Perform surgical organ retrieval following standard procurement protocols. Obtain the kidney graft and prepare for ex vivo perfusion.

▶ 01:00
2
Prime the normothermic perfusion circuit

Prepare and prime the NEVKP circuit with appropriate perfusate, establish blood flow pathways, and eliminate air from the system prior to kidney attachment.

▶ 02:55
3
Connect kidney to NEVKP system

Attach the kidney graft to the primed normothermic perfusion circuit, establish vascular connections, and initiate controlled perfusion.

▶ 04:56
4
Monitor graft perfusion and viability

Record perfusion parameters including flow rate, pressure, and oxygen consumption to assess kidney graft function and suitability for transplantation.

▶ 06:10
💬 Comments coming soon