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Immunology JoVE (Open Access) Citable · DOI

Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination

Name: Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination
Uploaded: 2026-10-12
Duration: 8 min 13 s
Description: Learn an optimized interferon-gamma ELISpot assay to measure T-cell responses in guinea pigs after vaccination. This method enables characterization of cellular immunity without necropsy.

DOI: 10.3791/58595-v

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Inconsistent Results Between Wells (Edge Effects)

Results vary significantly between wells that should be replicates. Edge wells often show different spot counts or characteristics compared to central wells, creating systematic position-dependent effects.

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