Home Cell Biology P50 Sensory Gating in Infants
Cell Biology JoVE (Open Access) Citable · DOI

P50 Sensory Gating in Infants

DOI: 10.3791/50065-v
What you'll learn
  • Set up auditory P50 sensory gating stimulus presentation equipment
  • Prepare infant subjects and recording apparatus for EEG data collection
  • Record and analyze P50 auditory evoked potentials in infants
  • Interpret sensory gating as a marker of cerebral inhibition
Protocol

Biopharma Insights Methods to record auditory P50 sensory gating, a physiological marker of cerebral inhibition which reflects early stages of attention, are described.

Difficulty
advanced
Total time
~1–2 hours per infant session
Model organism
Human infants

Steps

1
Configure auditory stimulus presentation parameters

Set up stimulus delivery equipment and define auditory tone parameters (frequency, intensity, interstimulus intervals) required for P50 gating paradigm testing in infants.

▶ 03:15
2
Prepare infant and EEG recording setup

Position the infant subject, apply EEG electrode cap or individual electrodes, verify electrode impedance, and confirm all recording hardware connections before data acquisition begins.

▶ 04:58
3
Conduct P50 auditory evoked potential recording

Execute the paired-stimulus P50 gating protocol, presenting conditioning and test stimuli while recording continuous EEG activity from relevant scalp sites.

▶ 08:18
4
Analyze P50 amplitude and gating ratio data

Extract P50 component amplitudes from conditioning and test responses, calculate sensory gating ratio, and interpret results as a physiological index of inhibitory capacity.

▶ 09:37
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