This study describes classical hydration using the thin lipid film method for nanoliposome preparation followed by nanoparticle characterization. A 47 kDa-hydrophilic and globular protein, tarin, is successfully encapsulated as a strategy to improve stability, avoid fast clearance, and promote controlled release. The method can be adapted to hydrophobic molecules encapsulation.
Total time
~4-6 hours per batch (including drying, hydration, and initial characterization)
Steps
1
Prepare tarin liposomal nanocapsules via thin film hydration
Dissolve lipids in organic solvent, evaporate to form thin film, then hydrate with aqueous protein solution to generate nanoliposome suspension containing encapsulated tarin.
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2
Determine protein encapsulation efficiency percentage
Quantify amount of tarin trapped within liposomes versus total protein using appropriate analytical method to assess encapsulation success.
▶ 04:24
3
Measure nanoliposome size and long-term stability
Use dynamic light scattering or similar technique to determine particle diameter and monitor size changes over storage time.
▶ 05:58
4
Characterize liposome morphology via electron microscopy
Visualize nanoliposome structure and confirm spherical morphology using transmission or scanning electron microscopy.
▶ 06:31
5
Interpret encapsulation and characterization results
Compile and analyze data from efficiency, size, stability, and morphology measurements to validate successful nanoliposome preparation.
▶ 08:29