Home › Cell Biology › Preparation and Pathogen Inactivation of Double Dose Buffy Coat Platelet Products using the INTERCEPT Blood System
Cell Biology JoVE (Open Access) Citable · DOI

Preparation and Pathogen Inactivation of Double Dose Buffy Coat Platelet Products using the INTERCEPT Blood System

DOI: 10.3791/4414-v
What you'll learn
  • ✓ Prepare double dose buffy coat platelet concentrates from whole blood donations
  • ✓ Apply INTERCEPT Blood System pathogen inactivation to platelet products
  • ✓ Evaluate in vitro platelet quality metrics over 7-day storage period
  • ✓ Implement blood bank standard operating procedures for platelet processing
Protocol

This article describes the process used by Örebro University Hospital to produce double dose buffy coat platelet concentrates prepared from whole blood donations and treated with the INTERCEPT Blood System for pathogen inactivation. The in vitro quality of the final platelet units are evaluated over 7 days of storage.

Difficulty
advanced
Total time
~7 days (initial processing ~4 hours; quality analysis distributed over storage period)
Biosafety
BSL-2

Steps

1
Review schematic overview of platelet preparation workflow

Understand the complete process flow from whole blood donation through buffy coat preparation, pooling, pathogen inactivation, and quality assessment.

▶ 00:28
2
Prepare buffy coat layer from whole blood donation

Isolate the buffy coat containing platelets and white blood cells from centrifuged whole blood units using standard blood bank separation techniques.

▶ 02:05
3
Pool multiple buffy coat units for concentration

Combine selected buffy coat fractions to create double dose platelet concentrates meeting target platelet count and volume specifications.

▶ 03:05
4
Inactivate pathogens using INTERCEPT Blood System

Treat pooled platelet products with amotosalen and ultraviolet A light to inactivate bacteria, viruses, and other pathogens while preserving platelet function.

▶ 05:07
5
Analyze stored platelet quality over seven days

Perform in vitro testing of platelet viability, function, and bacterial contamination at defined timepoints during storage to verify product safety and efficacy.

▶ 08:59
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