Cell Biology JoVE (Open Access) Citable · DOI

Preparation of 2-dGuo-Treated Thymus Organ Cultures

Name: Preparation of 2-dGuo-Treated Thymus Organ Cultures
Uploaded: 2026-06-17
Duration: 7 min 50 s
Description: In the thymus, interactions between developing T-cell precursors and stromal cells that include cortical and medullary epithelial cells are known to play ...

DOI: 10.3791/906-v

What you'll learn

✓ Dissect and isolate fetal thymus tissue from mouse embryos
✓ Prepare ex vivo thymus organ cultures treated with 2-dGuo
✓ Culture alymphoid thymus tissue for immunological studies

Protocol

This video demonstrates the dissection and removal of the fetal thymus as well the preparation of ex vivo cultures of 2-dGuo-treated thymus.

Difficulty

intermediate

Total time

~1–2 hours per litter (dissection and culture setup)

Model organism

Mouse (fetal)

Biosafety

BSL-1

Steps

Understand 2-dGuo thymus culture methodology

Review the scientific rationale for 2-dGuo treatment in thymus organ cultures and its application to studying lymphocyte development in alymphoid tissues.

▶ 00:15

Prepare and culture 2-dGuo-treated thymus

Dissect fetal thymus tissue, treat with 2-dGuo to create alymphoid conditions, and establish ex vivo organ cultures suitable for immunological analysis.

▶ 01:09

Summarize protocol and applications

Review the completed protocol and discuss the utility of 2-dGuo-treated thymus cultures for investigating lymphocyte development and immune reconstitution.

▶ 06:50

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