Cell Biology JoVE (Open Access) Citable · DOI

Preparation of Single-Cell Suspensions from Mouse Spleen with the gentleMACS Dissociator

Name: Preparation of Single-Cell Suspensions from Mouse Spleen with the gentleMACS Dissociator
Uploaded: 2026-06-23
Duration: 5 min 18 s
Description: Single-cell suspensions are a prerequisite for experiments in cell separation, cell analysis and cell culture. To avoid tedious and often painful manual ...

DOI: 10.3791/1029-v

What you'll learn

✓ Operate the gentleMACS Dissociator to automate tissue dissociation
✓ Prepare single-cell suspensions from mouse spleen tissue
✓ Filter and process dissociated splenocytes for downstream analysis

Protocol

This video describes a simple, time-saving technique for automated tissue dissociation using the gentleMACS Dissociator to prepare single-cell suspensions of mouse splenocytes.

Difficulty

beginner

Total time

~15–20 min per spleen

Model organism

Mouse

Biosafety

BSL-1

Steps

Introduce gentleMACS Dissociator equipment and setup

Familiarize with the gentleMACS Dissociator device, its components, and operational capabilities for automated tissue dissociation.

▶ 00:58

Dissociate mouse spleen using gentleMACS protocol

Load prepared spleen tissue into the gentleMACS Dissociator and run the automated dissociation program to generate single-cell suspension.

▶ 01:55

Filter dissociated spleen suspension through strainer

Pass the dissociated spleen cell suspension through a filter to remove debris and obtain a clean single-cell preparation.

▶ 03:42

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