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Microbiology JoVE (Open Access) Citable · DOI

Protocols for Oral Infection of Lepidopteran Larvae with Baculovirus

DOI: 10.3791/888-v
What you'll learn
  • ✓ Perform droplet feeding bioassay for baculovirus infection in lepidopteran larvae
  • ✓ Execute diet plug bioassay methodology to assess viral insecticidal efficiency
  • ✓ Evaluate and compare two oral infection techniques for biopesticide screening
Protocol

Biopharma Insights In this video, we demonstrate oral infection techniques of lepidopteran larvae with baculovirus in order to determine insecticidal efficiency.

Difficulty
intermediate
Total time
~3–5 days per bioassay (infection to mortality assessment)
Model organism
Lepidopteran larvae (Spodoptera species or equivalent pest insect)
Biosafety
BSL-1

Steps

1
Introduction to baculovirus oral infection protocols

Review the rationale for testing baculovirus insecticidal efficiency via oral routes and establish the context for lepidopteran larvae bioassays.

▶ 01:05
2
Perform droplet feeding bioassay with larvae

Apply baculovirus suspension as droplets on host plant material or directly onto larvae, then monitor infection and mortality endpoints.

▶ 01:41
3
Execute diet plug bioassay infection method

Prepare artificial diet plugs inoculated with baculovirus, expose larvae to contaminated plugs, and record infection kinetics and survival.

▶ 05:37
4
Compare and interpret bioassay results

Summarize findings from both infection routes, discuss relative advantages of droplet versus diet plug methods for efficacy screening.

▶ 07:37
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