✓Prepare PuraMatrix gel for cancer cell encapsulation
✓Suspend cancer cells for optimal encapsulation efficiency
✓Encapsulate and culture cancer cells in PuraMatrix scaffold
✓Evaluate cell viability and growth within peptide gel
Protocol
This video demonstrates how to encapsulate and culture cancer cells in PuraMatrix, a commercially available self assembling peptide gel.
Difficulty
intermediate
Total time
~3-4 hours (including gel preparation and cell encapsulation; cultures observed over days)
Model organism
Cancer cell lines (specific type not specified)
Biosafety
BSL-2
Steps
1
Understand PuraMatrix self-assembling peptide gel
Review the principles and properties of PuraMatrix as a commercially available self-assembling peptide scaffold for three-dimensional cell culture applications.
▶ 00:47
2
Prepare PuraMatrix gel for cell encapsulation
Reconstitute and prepare the PuraMatrix solution according to manufacturer instructions to achieve proper gelation conditions before cell seeding.
▶ 01:17
3
Prepare cancer cell suspension for encapsulation
Harvest and prepare cancer cells at appropriate concentration and viability for homogeneous distribution within the PuraMatrix scaffold.
▶ 02:56
4
Encapsulate cells within PuraMatrix gel
Combine prepared cell suspension with PuraMatrix solution and allow gelation to occur, creating a three-dimensional culture microenvironment.
▶ 04:06
5
Observe encapsulated cell growth and viability
Culture and monitor encapsulated cancer cells over time, documenting cell morphology, proliferation, and viability within the peptide gel matrix.
▶ 06:04
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