Home › Cell Biology › Rapid Genetic Analysis of Epithelial-Mesenchymal Signaling During Hair Regeneration
Cell Biology JoVE (Open Access) Citable · DOI

Rapid Genetic Analysis of Epithelial-Mesenchymal Signaling During Hair Regeneration

DOI: 10.3791/4344-v
What you'll learn
  • ✓ Isolate and prepare primary dermal and epidermal cells from mouse skin tissue
  • ✓ Perform lentiviral-mediated gain- or loss-of-function genetic modification in hair follicle cells
  • ✓ Graft modified epithelial and mesenchymal cell populations to assess hair regeneration
  • ✓ Conduct histological analysis to evaluate hair follicle growth and morphogenesis
Protocol

Biopharma Insights Tissue-specific analysis of a hair follicle regeneration model using lentivirus to mediate gain- or loss-of-function.

Difficulty
advanced
Total time
~7–10 days (including viral transduction, cell culture expansion, graft integration, and histological processing)
Model organism
Mouse (strain not specified)
Biosafety
BSL-2

Steps

1
Isolate and prepare mouse skin tissue

Remove dorsal skin from mouse and prepare tissue for cell isolation. Includes cleaning, trimming, and initial dissociation steps.

▶ 01:31
2
Isolate primary dermal and epidermal cells

Separate dermis from epidermis and enzymatically dissociate each layer into single-cell suspensions for culture and genetic modification.

▶ 03:17
3
Graft modified dermal and epidermal cells

Recombine lentivirally transduced dermal and epidermal cells and implant the construct onto recipient mice to initiate hair follicle regeneration.

▶ 05:07
4
Perform histological analysis of hair follicles

Harvest grafted skin, process tissue sections, and conduct microscopic evaluation to assess hair follicle growth, morphology, and epithelial-mesenchymal signaling.

▶ 07:57
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