Biochemistry JoVE (Open Access) Citable · DOI

Rapid Point-of-Care Assay of Enoxaparin Anticoagulant Efficacy in Whole Blood

Name: Rapid Point-of-Care Assay of Enoxaparin Anticoagulant Efficacy in Whole Blood
Uploaded: 2026-06-17
Duration: 11 min 17 s
Description: There is the need for a clinical assay to determine the extent to which a patient's blood is effectively anticoagulated by the ...

DOI: 10.3791/3852-v

What you'll learn

✓ Perform rapid quantitative assessment of enoxaparin anticoagulant effect in whole blood
✓ Apply heparinase digestion to isolate and measure low-molecular-weight-heparin contribution
✓ Operate point-of-care coagulation assay apparatus and interpret results

Protocol

Demonstration of a rapid quantitative assay of the inhibition of blood coagulation by the low-molecular-weight-heparin, enoxaparin. The contribution of enoxaparin is assessed by removing its influence through digestion with heparinase. A fuller description of the assay is detailed in our published paper.1 The assay still requires clinical confirmation.

Difficulty

intermediate

Total time

~15-20 min/sample

Biosafety

BSL-1

Steps

Understand enoxaparin efficacy assay principle

Review the fundamentals of measuring low-molecular-weight-heparin inhibition of blood coagulation and the rationale for heparinase-based removal of enoxaparin influence to quantify its contribution.

▶ 00:05

Identify assay apparatus and equipment

Familiarize with the point-of-care device and instrumental setup required for rapid quantitative coagulation assessment.

▶ 02:25

Digest samples with heparinase enzyme

Prepare whole blood samples and treat with heparinase to selectively degrade enoxaparin, enabling quantification of its anticoagulant effect.

▶ 02:39

Load and prepare assay cartridges

Insert processed blood samples into assay cartridges according to manufacturer specifications for point-of-care testing.

▶ 05:25

Execute quantitative coagulation assay procedure

Run the complete assay protocol on the point-of-care apparatus, monitoring blood coagulation kinetics with and without heparinase treatment.

▶ 06:21

Analyze and interpret assay results

Review quantitative output data to determine enoxaparin efficacy based on differences in coagulation parameters between heparinase-treated and untreated samples.

▶ 09:22

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