Home › Neuroscience › Recording Large-scale Neuronal Ensembles with Silicon Probes in the Anesthetized Rat
Neuroscience JoVE (Open Access) Citable · DOI

Recording Large-scale Neuronal Ensembles with Silicon Probes in the Anesthetized Rat

DOI: 10.3791/3282-v
What you'll learn
  • ✓ Prepare anesthetized rat for surgery
  • ✓ Implant silicon probes into brain
  • ✓ Record extracellular neural activity
  • ✓ Analyze multi-neuron ensemble data
Protocol

Biopharma Insights Extracellular recordings of neuronal activity using silicon probes in the anesthetized rat will be described. This technique allows information to be obtained across multiple brain areas from more than 100 neurons simultaneously. It provides information with single cell resolution about neuronal ensembles dynamics in multiple local circuits.

Difficulty
advanced
Total time
~3-4 hrs per animal
Model organism
Rat
Biosafety
BSL-1

Steps

1
Prepare rat for surgery

Anesthetize the rat and position it in a stereotaxic frame; expose the skull for craniotomy.

▶ 01:45
2
Implant silicon probes

Lower the silicon probes into the brain targeting regions of interest for extracellular recordings.

▶ 02:53
3
Record large-scale neuronal ensembles

Acquire extracellular signals from multiple brain areas simultaneously with high temporal resolution.

▶ 04:27
4
Review conclusion and results

Summarize the representative data and discuss implications for studying neuronal ensemble dynamics.

▶ 05:10
💬 Comments coming soon