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Immunology JoVE (Open Access) Citable · DOI

Registered Bioimaging of Nanomaterials for Diagnostic and Therapeutic Monitoring

DOI: 10.3791/2459-v
What you'll learn
  • ✓ Prepare nanomaterials for in vivo imaging
  • ✓ Perform animal preparation for imaging
  • ✓ Acquire and analyze biodistribution data
  • ✓ Correlate imaging with histological results
Protocol

Biopharma Insights Bioimaging methods used to assess cell biodistribution of nanoparticles are applicable for therapeutic and diagnostic monitoring of nanoformulated compounds. The methods described herein are sensitive and specific when assessed by histological coregistration. The methodologies provide a translational pathway from rodent to human applications.

Difficulty
intermediate
Total time
~2-3 days including animal prep and imaging
Model organism
Rodent (mouse or rat)
Biosafety
BSL-1

Steps

1
Prepare nanomaterial formulations

Synthesize or label nanoparticles with imaging agents for subsequent injection.

▶ 04:05
2
Prepare animals for imaging

Anesthetize and position the animal to ensure stable imaging conditions.

▶ 05:26
3
Acquire imaging data

Use imaging system to collect signal from nanoparticles in vivo over time.

▶ 07:44
4
Analyze acquired imaging data

Process images to quantify nanoparticle distribution and signal intensity.

▶ 11:10
5
Review example imaging results

Compare observed results with representative examples for validation.

▶ 14:12
6
Conclude with translational implications

Summarize findings and discuss relevance for diagnostic and therapeutic monitoring.

▶ 15:06
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