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Biochemistry JoVE (Open Access) Citable · DOI

Residue-specific Incorporation of Noncanonical Amino Acids into Model Proteins Using an Escherichia coli Cell-free Transcription-translation System

Name: Residue-specific Incorporation of Noncanonical Amino Acids into Model Proteins Using an Escherichia coli Cell-free Transcription-translation System
Uploaded: 2026-10-13
Duration: 11 min 47 s
Description: Learn to incorporate noncanonical amino acids into model proteins using an Escherichia coli cell-free transcription-translation system. This method enables toxic amino acid incorporation without living hosts for structural biology research.

DOI: 10.3791/54273-v

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