Biopharma Insights Creating experimental retinal detachments with a reproducible and sustained height of detachment, and without subretinal hemorrhage, is important for studying the pathophysiology of photoreceptor cell loss in retinal disease and evaluating potential therapeutic interventions. Here, we report such a method in detail.
Total time
~30-45 min/animal plus recovery
Model organism
Rodent (e.g., mouse or rat)
Steps
1
Prepare and anesthetize rodent for surgery
Anesthetize the rodent and position it under the microscope for subretinal injection.
▶ 02:17
2
Inject sodium hyaluronate subretinally
Perform subretinal injection of sodium hyaluronate to induce a controlled retinal detachment.
▶ 04:42
3
Assess retinal detachment time course
Observe and document the progression of retinal detachment over time.
▶ 05:16