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Analytical Chem JoVE (Open Access) Citable · DOI

Sexual Development and Ascospore Discharge in Fusarium graminearum

DOI: 10.3791/3895-v
What you'll learn
  • ✓ Perform sexual crosses to generate F. graminearum perithecia
  • ✓ Conduct ascospore discharge assays to measure spore release
  • ✓ Isolate and culture recombinant progeny from sexual crosses
  • ✓ Evaluate typical morphological outcomes of mating protocols
Protocol

Sexual crosses and isolation of recombinant progeny are important research tools for the filamentous fungus, Fusarium graminearum, The techniques necessary successfully carry out these processes are presented.

Difficulty
intermediate
Total time
~2–3 weeks (including incubation and isolation periods)
Model organism
Fusarium graminearum
Biosafety
BSL-1

Steps

1
Produce perithecia from sexual crosses

Establish mating between compatible F. graminearum strains on appropriate media to induce formation of fruiting bodies (perithecia) containing developing ascospores.

▶ 01:03
2
Perform ascospore discharge assay

Quantify viable ascospore release from mature perithecia using collection plates or specialized apparatus to measure spore viability and discharge dynamics.

▶ 02:46
3
Generate and isolate recombinant progeny

Collect discharged ascospores, culture individual spore-derived colonies, and select recombinant isolates based on phenotypic or genotypic markers from the sexual cross.

▶ 03:53
4
Evaluate typical culturing results

Assess morphological and growth characteristics of perithecia and recombinant colonies to confirm successful sexual development and progeny isolation.

▶ 06:41
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