Home › Analytical Chem › Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples
Analytical Chem JoVE (Open Access) Citable · DOI

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples

DOI: 10.3791/54535-v
What you'll learn
  • ✓ Set up a sheathless CE-MS system with porous tip interface design
  • ✓ Prepare and analyze metabolite standards for system calibration
  • ✓ Perform metabolic profiling on biological samples using CE-MS
Protocol

A protocol for metabolic profiling of biological samples by capillary electrophoresis–mass spectrometry using a sheathless porous tip interface design is presented.

Difficulty
advanced
Total time
~4–8 hours per sample batch (including system setup, calibration, and analysis)

Steps

1
Assemble sheathless CE-MS porous tip interface

Configure the capillary electrophoresis–mass spectrometry system with a sheathless porous tip interface design. Connect fluidic and electrical components according to manufacturer specifications.

▶ 01:50
2
Analyze metabolite standards and optimize parameters

Run metabolite standard solutions through the CE-MS system to establish baseline separation, detection sensitivity, and method optimization prior to biological sample analysis.

▶ 04:31
3
Profile metabolites in biological samples

Apply the optimized CE-MS method to analyze prepared biological samples, generating metabolic profiles and quantitative data for downstream interpretation.

▶ 06:21
💬 Comments coming soon