Home › Cell Biology › Subcutaneous Angiotensin II Infusion using Osmotic Pumps Induces Aortic Aneurysms in Mice
Cell Biology JoVE (Open Access) Citable · DOI

Subcutaneous Angiotensin II Infusion using Osmotic Pumps Induces Aortic Aneurysms in Mice

DOI: 10.3791/53191-v
What you'll learn
  • ✓ Fill and prepare osmotic pumps with angiotensin II for subcutaneous implantation
  • ✓ Implant osmotic pumps subcutaneously in mice using aseptic surgical technique
  • ✓ Assess aortic aneurysm formation via imaging in angiotensin II-infused mice
Protocol

Subcutaneous implantation of osmotic pumps provides a convenient approach for prolonged and consistent delivery of compounds. This approach has been used extensively to study both abdominal and thoracic aortic aneurysms in mice.

Difficulty
advanced
Total time
~45 min per mouse implantation; aneurysm development requires 28 days post-implantation
Model organism
Mouse (strain not specified, commonly C57BL/6J)
Biosafety
BSL-1

Steps

1
Fill osmotic pumps with angiotensin II solution

Prepare osmotic pumps by loading with angiotensin II compound. This ensures consistent drug delivery over the implantation period.

▶ 00:59
2
Implant osmotic pump subcutaneously in mouse

Surgically place the filled osmotic pump in the subcutaneous space of the anesthetized mouse using sterile technique. Secure incision and allow recovery.

▶ 03:45
3
Image aorta to document aneurysm formation

Use ultrasound, CT, or other imaging modality to visualize and measure aortic changes in angiotensin II-infused mice following implantation period.

▶ 05:45
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