Biopharma Insights Mesh electronics probes seamlessly integrate and provide stable, long-term, single-neuron level recording within the brain. This protocol uses mesh electronics for in vivo experiments, involving the fabrication of mesh electronics, loading into needles, stereotaxic injection, input/output interfacing, recording experiments, and histology of tissue containing mesh probes.
Total time
~4–6 hours per mouse (including surgery, setup, and recording session)
Model organism
Mouse (strain not specified)
Steps
1
Fabricate mesh electronics probes
Construct syringe-injectable mesh electronics using microfabrication techniques to create flexible probes suitable for neural integration.
▶ 00:41
2
Load mesh electronics into injection needles
Carefully load fabricated mesh electronics into syringe needles while maintaining probe integrity and sterility for injection.
▶ 03:59
3
Stereotaxically inject mesh into mouse brain
Use stereotaxic surgery to precisely position and inject mesh electronics into target brain region of anesthetized mouse.
▶ 04:32
4
Interface input/output recording connections
Establish electrical connections between mesh electronics and external recording equipment for signal acquisition and data transmission.
▶ 06:16
5
Perform neural recording experiments
Conduct in vivo electrophysiology recordings to capture single-neuron activity from implanted mesh electronics.
▶ 08:06
6
Analyze representative neural recording data
Review and interpret recorded neural signals demonstrating stable, long-term single-unit activity from mesh-integrated probes.
▶ 08:25
7
Perform histology of mesh-implanted tissue
Process brain tissue post-mortem to visualize mesh probe integration and verify electrode placement within target region.
▶ 09:17