Home › Botany › Techniques for Processing Eyes Implanted with a Retinal Prosthesis for Localized Histopathological Analysis: Part 2 Epiretinal Implants with Retinal Tacks
Botany JoVE (Open Access) Citable · DOI

Techniques for Processing Eyes Implanted with a Retinal Prosthesis for Localized Histopathological Analysis: Part 2 Epiretinal Implants with Retinal Tacks

DOI: 10.3791/52348-v
What you'll learn
  • ✓ Remove and prepare epiretinal implants with retinal tacks for histological analysis.
  • ✓ Execute dehydration, embedding, and sectioning protocols for implanted ocular tissue.
  • ✓ Perform staining and imaging to visualize tissue-implant interfaces at multiple magnifications.
Protocol

Here we describe histological techniques for visualising ocular tissue directly adjacent to a metal epiretinal tack and retinal prosthesis.

Difficulty
advanced
Total time
~3–4 days per eye (including dehydration and embedding time)

Steps

1
Remove electrode and dissect implanted eye

Carefully extract the epiretinal prosthesis and retinal tack from the implanted eye, then perform gross dissection to isolate the region of interest containing the retinal tissue adjacent to the implant.

▶ 02:15
2
Dehydrate, embed, mount, and grind sample

Process the dissected tissue through graded ethanol dehydration, infiltrate with embedding medium, mount the specimen, and perform grinding to create flat sections suitable for microscopy.

▶ 04:18
3
Stain and image tissue-implant interface

Apply histological staining to the ground sections and acquire high- and low-power microscopic images to visualize the retinal tissue and surrounding structures in contact with the retinal tack.

▶ 07:51
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