Home › Cell Biology › The Bovine Lung in Biomedical Research: Visually Guided Bronchoscopy, Intrabronchial Inoculation and In Vivo Sampling Techniques
Cell Biology JoVE (Open Access) Citable · DOI

The Bovine Lung in Biomedical Research: Visually Guided Bronchoscopy, Intrabronchial Inoculation and In Vivo Sampling Techniques

DOI: 10.3791/51557-v
What you'll learn
  • ✓ Perform bronchoscopic guided inoculation in bovine lungs
  • ✓ Execute bronchoalveolar lavage and bronchial brushing techniques
  • ✓ Collect transbronchial lung biopsy samples under visual guidance
  • ✓ Prepare and process respiratory samples for downstream analysis
Protocol

This article describes bronchoscopic techniques in the bovine lung under experimental conditions, i.e. bronchoscopically guided inoculation, bronchoalveolar lavage, bronchial brushing, and transbronchial lung biopsy.

Difficulty
advanced
Total time
~2-3 hours per animal (bronchoscopy procedure with sample collection and processing)
Model organism
Cattle (bovine)
Biosafety
BSL-2

Steps

1
Perform bronchoscopic guided intrabronchial inoculation

Use visual bronchoscopy guidance to deliver inoculant directly into target airways. This technique enables controlled experimental infection or substance delivery within the bovine respiratory tract.

▶ 01:39
2
Collect bronchoalveolar lavage fluid samples

Perform bronchoalveolar lavage (BALF) to obtain cellular and biochemical samples from distal airways and alveoli. This non-terminal sampling method recovers immune cells and soluble mediators from lung compartments.

▶ 03:32
3
Obtain bronchial brush samples from airways

Use bronchoscopic brushing to collect epithelial cells and microorganisms from bronchial surfaces. Brushings capture airway-specific cellular material for microbial, immunological, or histological analysis.

▶ 04:44
4
Obtain transbronchial lung tissue biopsy

Collect small lung tissue samples via bronchoscopic biopsy forceps under direct visualization. Transbronchial biopsies provide histopathological assessment of parenchymal and airway pathology.

▶ 06:05
5
Prepare and process collected respiratory samples

Process bronchoalveolar lavage, brush, and biopsy samples for downstream applications including cell counting, culturing, staining, and molecular analysis. Proper sample handling preserves sample integrity for accurate results.

▶ 07:21
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