Cell Biology JoVE (Open Access) Citable · DOI

Tomato Analyzer: A Useful Software Application to Collect Accurate and Detailed Morphological and Colorimetric Data from Two-dimensional Objects

Name: Tomato Analyzer: A Useful Software Application to Collect Accurate and Detailed Morphological and Colorimetric Data from Two-dimensional Objects
Uploaded: 2026-06-17
Duration: 15 min 25 s
Description: Measuring fruit morphology and color traits of vegetable and fruit crops in an objective and reproducible way is important for detailed phenotypic ...

DOI: 10.3791/1856-v

What you'll learn

✓ Acquire high-quality digitalized images of tomato fruit for morphological analysis
✓ Use Tomato Analyzer software to quantify shape and color attributes reproducibly
✓ Export and apply morphological and colorimetric data for research applications

Protocol

Tomato Analyzer (TA) quantifies attributes of two dimensional shapes and color in a reproducible and accurate manner. A step-by-step procedure for obtaining high quality digitalized images of tomato fruit, morphological and color analyses of these images and several applications using the data generated through this software are described.

Difficulty

beginner

Total time

~1–2 hours per batch of fruit (image acquisition and analysis)

Model organism

Solanum lycopersicum (tomato)

Steps

Introduce Tomato Analyzer software capabilities

Overview of Tomato Analyzer's ability to quantify two-dimensional shape and color attributes in a reproducible and accurate manner for morphological and colorimetric analyses.

▶ 00:40

Select and prepare plant material for imaging

Choose tomato fruit and prepare samples for digitalization, ensuring consistent presentation for downstream image analysis.

▶ 01:16

Collect high-quality digitalized fruit images

Acquire standardized digital images of tomato fruit using proper imaging techniques and calibration to ensure reproducible morphological and color data.

▶ 02:26

Perform image analysis and color calibration

Use Tomato Analyzer to analyze fruit morphology and execute color calibration procedures for accurate colorimetric quantification.

▶ 05:08

Save and export analysis data

Output morphological and colorimetric data from Tomato Analyzer in formats suitable for downstream statistical and comparative applications.

▶ 12:20

Apply quantified data to research questions

Demonstrate representative results and practical applications of morphological and colorimetric data generated through Tomato Analyzer.

▶ 14:23

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