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Cell Biology JoVE (Open Access) Citable · DOI

Transdermal Measurement of Glomerular Filtration Rate in Mice

DOI: 10.3791/58520-v
What you'll learn
  • ✓ Prepare mice and transdermal GFR monitor for noninvasive kidney function assessment
  • ✓ Attach transdermal monitor to conscious, freely moving mice
  • ✓ Inject FITC-sinistrin tracer and acquire real-time GFR measurements
  • ✓ Interpret representative GFR monitoring data and results
Protocol

Here we describe a protocol to measure glomerular filtration rate (GFR) in conscious, freely moving mice using a transdermal GFR monitor.

Difficulty
intermediate
Total time
~45 min per mouse (single measurement cycle)
Model organism
Mouse
Biosafety
BSL-1

Steps

1
Prepare mouse and transdermal GFR monitor

Acclimate the mouse and calibrate the transdermal GFR monitor device according to manufacturer specifications. Ensure all monitoring equipment is functioning properly before animal attachment.

▶ 01:14
2
Attach transdermal monitor to conscious mouse

Position the transdermal GFR monitor on the mouse skin in the designated measurement area. Secure the device to allow continuous contact while permitting normal movement.

▶ 02:18
3
Inject FITC-sinistrin tracer and initiate measurement

Administer FITC-sinistrin intravenously to the conscious mouse and activate transdermal monitoring. Collect real-time fluorescence data as the tracer is filtered through the glomerulus.

▶ 03:26
4
Analyze representative GFR monitoring results

Review the acquired transdermal GFR measurements and interpret the resulting kinetic curves to calculate glomerular filtration rate values from the monitoring data.

▶ 05:11
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