Home › Microbiology › Treatment of Platelet Products with Riboflavin and UV Light: Effectiveness Against High Titer Bacterial Contamination
Microbiology JoVE (Open Access) Citable · DOI

Treatment of Platelet Products with Riboflavin and UV Light: Effectiveness Against High Titer Bacterial Contamination

DOI: 10.3791/52820-v
What you'll learn
  • ✓ Prepare bacterial suspensions and measure titer using colony forming assays
  • ✓ Apply riboflavin and UV light pathogen reduction to platelet products
  • ✓ Evaluate bacterial inactivation effectiveness in contaminated platelet samples
Protocol

The storage conditions of platelet products create an ideal environment for bacterial contaminants to multiply to dangerous levels. The goal of this study was to use a colony forming assay to evaluate a riboflavin based pathogen reduction process against high titer bacterial contamination in human platelet products.

Difficulty
intermediate
Total time
~4–6 hours (bacterial preparation, titer enumeration, treatment, and assay incubation)
Biosafety
BSL-2

Steps

1
Prepare bacterial suspension from culture

Culture and prepare a standardized bacterial suspension for contamination inoculation into platelet products. This suspension will be used to spike samples to high titer levels.

▶ 02:28
2
Determine bacterial titer by colony count

Perform serial dilutions and plate the bacterial suspension on growth media to quantify colony forming units per milliliter, establishing baseline contamination levels.

▶ 03:29
3
Prepare platelet product for treatment

Obtain and prepare human platelet products, then inoculate with the high-titer bacterial suspension to simulate contamination scenarios.

▶ 04:48
4
Apply riboflavin and UV light treatment

Expose inoculated platelet products to riboflavin and UV light according to the pathogen reduction protocol to inactivate bacterial contaminants.

▶ 05:47
5
Assess bacterial inactivation and results

Culture treated platelet samples and perform colony forming assays to quantify surviving bacteria and evaluate the effectiveness of the pathogen reduction process.

▶ 08:34
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