Home › Immunology › Ultrasound-Guided Orthotopic Implantation of Murine Pancreatic Ductal Adenocarcinoma
Immunology JoVE (Open Access) Citable · DOI

Ultrasound-Guided Orthotopic Implantation of Murine Pancreatic Ductal Adenocarcinoma

DOI: 10.3791/60497-v
What you'll learn
  • ✓ Perform ultrasound-guided orthotopic implantation of murine pancreatic cancer cells
  • ✓ Reduce peritoneal seeding compared to surgical implantation methods
  • ✓ Monitor tumor development via ultrasound imaging within 2–4 weeks
  • ✓ Apply image-guided injection techniques for precise pancreatic tumor placement
Protocol

We describe a protocol for the ultrasound guided implantation of murine-derived pancreatic ductal adenocarcinoma cell lines directly into the native tumor site. This approach resulted in pancreatic tumors detectable by ultrasound scanning within 2-4 weeks of injection, and significantly reduced the proportion tumor cell seeding on the peritoneal wall as compared to surgical orthotopic implantation.

Difficulty
advanced
Total time
~45–60 min per mouse (anesthesia, imaging, injection, recovery)
Model organism
Mouse (murine pancreatic ductal adenocarcinoma cell lines)
Biosafety
BSL-2

Steps

1
Prepare mouse for ultrasound-guided surgical procedure

Anesthetize the mouse and position for imaging access. Prepare the surgical site and ensure proper anesthesia monitoring throughout the procedure.

▶ 01:08
2
Perform ultrasound-guided pancreatic cancer cell implantation

Use ultrasound imaging to visualize the pancreas and guide needle placement for direct injection of murine-derived PDA cell lines into the native tumor site.

▶ 02:17
3
Assess tumor establishment via ultrasound imaging

Monitor implanted pancreatic tumors using ultrasound scanning to confirm successful orthotopic engraftment and measure tumor development over 2–4 weeks post-injection.

▶ 04:28
4
Interpret outcomes and procedural efficacy

Review results demonstrating reduced peritoneal wall seeding and reliable tumor detection compared to traditional surgical orthotopic implantation methods.

▶ 05:50
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