Use of a Robot for High-throughput Crystallization of Membrane Proteins in Lipidic Mesophases

Set up crystallization plates with appropriate well geometry and establish baseline conditions for mesophase-based crystallization experiments. This foundational step ensures proper sample delivery and consistent results.

Use the In Meso Robot to automatically dispense protein-laden lipidic mesophase into crystallization wells. This platform handles viscous mesophase materials with precision for high-throughput screening.

Prepare the robot syringe by priming with mesophase material to ensure consistent delivery rates and eliminate air bubbles prior to crystallization runs.

Run the programmed robotic protocol to dispense samples across multiple wells with controlled volumes and timing. Monitor system performance throughout the run.

Apply appropriate sealing methods to the crystallization plate to prevent evaporation and contamination during crystal growth incubation periods.

Operate the Mosquito LCP platform as an alternative high-throughput robotic system for lipidic cubic phase crystallization with comparable automation and throughput.

Use the Gryphon LCP platform, a third robotic option, for automated membrane protein crystallization in lipidic mesophases with distinct operational features.