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Cell Biology JoVE (Open Access) Citable · DOI

Use of High-Throughput Automated Microbioreactor System for Production of Model IgG1 in CHO Cells

DOI: 10.3791/58231-v
What you'll learn
  • ✓ Operate a 48-parallel automated microbioreactor system for mammalian cell culture
  • ✓ Execute seed train expansion and system preparation workflows
  • ✓ Harvest cell cultures and quantify IgG antibody titers
Protocol

A detailed protocol for the concurrent operation of 48 parallel cell cultures under varied conditions in a microbioreactor system is presented. Cell culture process, harvest and subsequent antibody titer analysis are described.

Difficulty
advanced
Total time
~7–10 days (including seed train expansion, microbioreactor run, and harvest)
Model organism
CHO cells (Chinese Hamster Ovary)
Biosafety
BSL-1

Steps

1
Expand seed train and prepare microbioreactor system

Culture CHO cells through seed train expansion and configure the automated 48-well microbioreactor platform with required media, inoculants, and sensors for parallel operation under varied conditions.

▶ 00:46
2
Initiate microbioreactor software and culture runs

Launch microbioreactor control software, inoculate wells with seed cultures, and begin automated monitoring of 48 parallel cell cultures under specified growth parameters.

▶ 02:55
3
Perform system shutdown and final data logging

Execute controlled shutdown of microbioreactor hardware, save experimental data, and document run parameters prior to harvest.

▶ 05:15
4
Harvest cultures and measure IgG antibody titers

Collect culture supernatants from microbioreactor wells, clarify samples, and quantify model IgG1 antibody concentrations using titer assays (e.g., ELISA or comparable method).

▶ 05:58
5
Analyze and interpret high-throughput results

Review representative data outputs from parallel microbioreactor analyses, including growth kinetics, productivity metrics, and process parameter effects on IgG production.

▶ 07:04
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