Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation

Seed engineered CHO cells expressing single chain MHC class I constructs into culture vessels at appropriate density to prepare them as antigen-presenting cells for downstream T cell activation assays.

Co-culture human CD8+ T cell clones with engineered CHO antigen-presenting cells and measure T cell activation parameters (e.g., cytokine production, proliferation) to assess responsiveness to single chain MHC-peptide complexes.

Label CHO cells with antibodies specific for MHC class I to verify surface expression of the engineered single chain constructs via flow cytometry or immunofluorescence.

Evaluate experimental results to characterize molecular interactions and co-stimulatory effects of single chain MHC class I complexes on human CD8+ T cell activation kinetics and potency.