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Analytical Chem JoVE (Open Access) Citable · DOI

Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution

Name: Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution
Uploaded: 2026-10-23
Duration: 11 min 37 s
Description: Learn how to optimize CRISPR-Cas9 specificity using the Sniper-screen directed evolution method. This protocol identifies mutant Cas9 variants with reduced off-target effects while maintaining full on-target activity for therapeutic applications.

DOI: 10.3791/59202-v

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