The accompanying video describes the use of the GELFREE 8100 Fractionation System, which partitions complex protein samples on the basis of molecular weight and recovers the fractions in liquid phase. The video describes how the technology works, how it is used, and provides resultant data, with polyacrylamide gel electrophoresis analysis of fractionated bovine liver homogenate.
Total time
~3–4 hours per sample (including run time and fraction collection)
Steps
1
Understand GELFREE 8100 technology and workflow
Learn the principles of molecular weight-based protein fractionation using gel electrophoresis in a tube format with liquid-phase recovery, and review the complete experimental workflow.
▶ 00:11
2
Prepare protein samples for fractionation
Process and prepare complex protein samples (e.g., bovine liver homogenate) to appropriate concentration and format for loading into the GELFREE cartridge.
▶ 00:48
3
Load sample into GELFREE 8100 cartridge
Insert the prepared protein sample into the cartridge following the system's specifications to ensure proper fractionation during the run.
▶ 01:53
4
Run cartridge and collect liquid fractions
Execute the GELFREE 8100 separation program and systematically collect molecular weight-fractionated protein samples in liquid phase as they elute from the system.
▶ 03:00
5
Analyze fractions using polyacrylamide gel electrophoresis
Perform PAGE analysis on collected fractions to visualize protein composition by molecular weight and confirm successful fractionation.
▶ 06:37