✓Perform surgical preparation of mice for video-oculography measurement
✓Set up and calibrate video pupil tracking equipment for eye movement analysis
✓Collect and analyze quantitative ocular motor performance data
✓Interpret video-oculography results to assess motor learning and behavior
Protocol
Biopharma Insights Video-oculography is a very quantitative method to investigate ocular motor performance as well as motor learning. Here, we describe how to measure video-oculography in mice. Applying this technique on normal, pharmacologically-treated or genetically modified mice is a powerful research tool to explore the underlying physiology of motor behaviors.
Difficulty
advanced
Total time
~2-3 hours per mouse (including surgical recovery and measurement sessions)
Model organism
Mouse (unspecified strain)
Biosafety
BSL-1
Steps
1
Perform mouse surgery for video-oculography
Conduct surgical preparation of mice to enable video-oculography measurement. This involves preparation procedures necessary for subsequent ocular tracking.
▶ 01:14
2
Assemble and configure video-oculography equipment
Set up the video-oculography apparatus including optical and recording components. Position and align the system for accurate eye movement tracking.
▶ 02:51
3
Calibrate pupil tracking and record eye movements
Perform system calibration using video pupil tracking and execute baseline eye movement measurements. Verify tracking accuracy before data collection.
▶ 05:20
4
Process and analyze video-oculography data
Extract quantitative metrics from recorded eye movement videos. Compute parameters relevant to ocular motor performance and motor learning.
▶ 06:46
5
Interpret video-oculography results and outcomes
Review analyzed data to assess ocular motor performance and identify changes in motor behavior. Compare results across experimental conditions or genotypes.
▶ 07:42
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