Home›Microscopy & Imaging›Laser Confocal Microscope | How To Set Up Confocal Multicolor Experiments
Steps
1Introduce the Stellaris Confocal Microscope00:06
2Select Fluorophores for Your Sample00:25
3Assign Fluorophores to Detection Channels00:40
4Enable Live View and Verify Image Quality00:59
5Fine-Tune Imaging Parameters as Needed01:20
6Save Optimized Settings for Future Use01:32
Microscopy & ImagingLeica Microsystems
Laser Confocal Microscope | How To Set Up Confocal Multicolor Experiments
Protocol
Difficulty
intermediate
Steps
1
Introduce the Stellaris Confocal Microscope
The video introduces the Stellaris confocal microscope system and its new Image Compass user interface, which reimagines the acquisition workflow for setting up multicolor fluorescence experiments.
▶ 00:06
2
Select Fluorophores for Your Sample
Identify and select the fluorophores present in your specimen from the list displayed at the top of the screen. The example demonstrates setting up an experiment with five fluorophores.
▶ 00:25
3
Assign Fluorophores to Detection Channels
Drag and drop each selected fluorophore into one of the available detection channels using the intuitive interface. Image Compass automatically optimizes excitation and detection parameters for the fluorophore combination.
▶ 00:40
4
Enable Live View and Verify Image Quality
Switch on the live view mode to display the captured image in real-time, showing rich detail, contrast, and information with minimal user training required.
▶ 00:59
5
Fine-Tune Imaging Parameters as Needed
Access the control panel to adjust gain and laser intensity settings for optimal results. Monitor changes in real-time through the live view display.
▶ 01:20
6
Save Optimized Settings for Future Use
Save the configured experimental parameters at any time to apply the same settings to other samples with identical preparation, increasing experimental efficiency.