Liquid chromatography

Learn why filtering samples before liquid chromatography is critical for maintaining column life and preventing instrument damage. Sample filtration ensures optimal chromatographic run quality and prevents costly equipment failures.

Filter out particulate matter to prevent column blockage and reduce back pressure on the LC system. The pore size and retention properties of the filter material must match the particle size of the column being used.

Select filter material based on chemical compatibility with solvents in your sample to minimize non-specific binding of analytes of interest. This ensures sample integrity and accurate chromatographic results.

Choose a filter unit size (syringe filter, 13mm, or 4mm) that minimizes sample losses through hold-up volume, particularly important for very small sample volumes. Proper filter unit selection preserves analyte quantity for reliable analysis.