Home›Microbiology›Microbes are ubiquitous - Dianne Newman (Cal Tech/HHMI)
Steps
1Examine pond scum microorganisms00:02
2Discuss historical metabolic significance00:42
3Observe stationary phase metabolites02:15
4Examine soil rhizosphere colonization03:22
5Document plant-associated microbe benefits04:04
MicrobiologyiBiology Techniques
Microbes are ubiquitous - Dianne Newman (Cal Tech/HHMI)
Protocol
Difficulty
intermediate
Steps
1
Examine pond scum microorganisms
Observe a pond water sample containing phototrophs and microorganisms, particularly purple phototrophs that perform photosynthesis using reduced compounds like sulfur, hydrogen, or iron instead of water.
▶ 00:02
2
Discuss historical metabolic significance
Explain how these photosynthetic microorganisms shaped Earth's geochemistry over time, contributing to atmospheric oxygen evolution and formation of banded iron deposits, demonstrating their importance for alternative energy research.
▶ 00:42
3
Observe stationary phase metabolites
Identify secondary metabolites and pigments excreted by microorganisms during stationary phase growth, noting that these compounds possess pharmaceutical and antibiotic properties valuable to industry.
▶ 02:15
4
Examine soil rhizosphere colonization
View microscope images of Pseudomonas bacteria colonizing tomato root seedlings and forming biofilms on root surfaces, demonstrating beneficial plant-microbe interactions in the soil environment.
▶ 03:22
5
Document plant-associated microbe benefits
Summarize the beneficial roles of rhizosphere microorganisms in protecting plants from fungal pathogens through biocontrol agents and fixing atmospheric nitrogen as natural fertilizer.
▶ 04:04
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