Real-time imaging with BD CellView™ Image Technology

Overview of BD CellView imaging technology and its advancement beyond traditional flow cytometry for single-cell analysis.

Describe how BD CellView uses scatter and fluorescent detectors to reveal internal and external spatial characteristics of cells without requiring a traditional microscope camera.

Explain how conventional flow cytometry passes fluorochrome-stained cells through laser beams one at a time, collecting signals as electronic pulses.

Detail how the blue laser beam is directed through an acousto-optic deflector and split into 100 separate beams that blink at different frequencies as cells pass through.

Describe how each beam generates a signal collected onto a photomultiplier tube, with mathematically distinguishable frequencies that are reconstructed to produce a complete cellular image.

Explain how traditional flow parameters like scatter and fluorescence are combined with image features such as eccentricity, intensity, and size, while a light loss detector produces bright field images through a pinhole.