Overview of the clonogenic assay as an in vitro cell survival assay that measures reproductive integrity and colony-forming potential of individual cells.
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2
Explain single cell isolation and plating
Discuss the methodology of isolating single cells and plating them under controlled experimental conditions to allow colony formation.
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3
Culture cells for colony growth period
Incubate plated cells in appropriate growth media for the designated culture period to allow colony development and expansion.
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4
Fix and stain formed colonies
Fix the cultured cells and apply staining protocols to visualize and identify the colonies that have formed.
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5
Count colonies and analyze results
Count the visible colonies and calculate survival rates and colony-forming efficiency to assess cell reproductive capacity.
▶ 03:50
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