Liquid-Liquid Separation

Clamp a separating funnel to a stand. Pour the reaction mixture from the round bottom flask into the separating funnel, which contains the product in organic solvent.

Add water through the top of the separating funnel. Observe the water dripping to the bottom and the formation of two immiscible layers that separate according to density, with organic solvent on top.

Cap the separating funnel and hold the stopper firmly in place. Invert the funnel, open the tap to release pressure, then shake vigorously. Repeat this process multiple times to ensure thorough mixing of the layers.

Allow the layers to settle and separate. Open the tap and drain the lower water layer into the separating funnel, stopping just before it reaches the bottom. Swirl gently to release any remaining material.

Run the organic layer (upper layer) from the separating funnel into a conical flask for collection.

Return the water layer to the separating funnel and add fresh solvent (such as ether) in roughly equal volume. Cap, invert, vent, and shake vigorously to extract any remaining product from the water layer.

Allow the layers to separate and settle. Open the tap and drain the upper organic layer into the original conical flask containing the first organic layer.