Gather and organize all necessary equipment and reagents for the migration assay procedure. Set up the workspace and ensure all components are ready for use.
▶ --:--
2
Set up migration chamber or insert
Assemble the migration chamber or prepare the transwell insert according to protocol specifications. Position the chamber in the culture plate and prepare the barrier membrane.
▶ 01:20
3
Prepare cell suspension and load chamber
Prepare the cell suspension at the appropriate concentration and load cells into the upper chamber of the migration insert. Ensure even distribution of cells across the membrane.
▶ 02:40
4
Add chemoattractant to lower chamber
Add the chemoattractant solution or appropriate stimulus medium to the lower chamber to establish the migration gradient. Ensure proper concentration for optimal cell migration.
▶ 04:00
5
Incubate for cell migration
Incubate the migration assay at appropriate temperature and time duration to allow cells to migrate through the membrane toward the chemoattractant. Monitor timing according to experimental protocol.