Learn that AAS measures element concentration in samples by analyzing how light interacts with matter. The difference in light intensity before and after interaction with the sample determines the element's concentration.
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Select and apply hollow cathode lamp
Use a hollow cathode lamp specific to the target element to shine light that corresponds to the element's absorption spectra. This ensures all light can be absorbed by the element of interest.
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Aspirate sample into flame
Aspirate the sample into a mixture of fuel and oxygen, then burn the sample. This combustion process atomizes the sample so individual atoms can interact with the light.
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Pass light through monochromator
After light passes through the flame where absorption occurs, filter it through a monochromator to isolate a single specific wavelength for analysis.
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Detect and amplify signal
Direct the filtered light through a photomultiplier to amplify the signal for accurate measurement and detection.
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Calculate concentration using Beer's Law
Use computer technology to apply Beer's Law (A = εbc) to the measured absorbance data, where A is absorbance, ε is molar absorptivity, b is path length, and c is concentration.
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Apply AAS to practical applications
Utilize AAS in food and beverage research, water analysis, clinical research, pharmaceutical analysis, and mining operations to determine element concentrations in various samples.
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