如何使用新型ChemiDoc MP成像系统进行化学发光蛋白质印迹成像

Press the green power button on the ChemiDoc MP instrument. When the home screen appears, tap to access the login menu and enter your username credentials or create a new user account.

Open the imager drawer and pull out the transilluminator. Clean the UV tray with DI water and a Kimwipe to ensure optimal imaging performance.

Apply substrate to the membrane and center it on the UV tray. Close the drawer and select chemiluminescence as the application under blots. Adjust image size and zoom as needed for your region of interest.

Configure exposure settings using either automatic band detection or manual exposure time entry. Optionally use signal accumulation mode to find ideal exposure. Tap the camera button to capture the image, which automatically saves to the gallery.

Capture a colorimetric image of the molecular weight marker to visualize band positions. Select your preferred chemiluminescence exposure and tap merge to combine the images for complete visualization.

Tap individual bands to view their relative fluorescence units and percent saturation values for quantification. Use the transform icon to adjust image appearance for visualization without affecting actual data.

Tap the send/save icon and select your desired file format (including Image Lab format for further analysis). Choose destination as USB drive or network and export with any custom notes or metadata.