Immunofluorescence IF Protocol and Confocal Imaging

severe

Red Blood Cell and Heme-Related Autofluorescence

Strong autofluorescence across multiple channels in blood-rich tissues such as spleen, liver, brain, bone marrow, and vascularized tumors. Heme and porphyrins dominate signal especially when tissue is not thoroughly perfused.

💡 4 · ✓ 5

moderate

Inconsistent Antigen Retrieval Results

Antigen retrieval (AR) performance varies unpredictably across sections and targets. Harsh AR needed to recover signal suggests overfixation. Epitope accessibility inconsistent even with standardized protocols.

💡 5 · ✓ 6