How to Use the New ChemiDoc MP for Protein Gel Imaging

Turn on the ChemiDoc MP by pressing the green button and wait for the home screen to appear. Tap the screen to access the login menu and create a new user account if needed.

Open the imager drawer and pull out the transilluminator. Clean the appropriate tray with DI water and a kimwipe, then place your gel or blot centered on the tray. Push the transilluminator back in and close the drawer.

Zoom into the desired imaging area and select the image size (small, medium, or large). Tap application to choose the stain type for your sample, then take a preview and set your region of interest.

Choose your exposure time manually or enable automatic band detection. Tap the camera button to capture the protein gel image with your chosen stain.

Place the Stain-Free gel directly on the UV tray and close the drawer. Select Stain-Free Gel as your application and set the activation time to 45 seconds for blotting or 5 minutes for increased sensitivity.

Tap individual bands to view relative fluorescence units and percent saturation values. Use the transform icon to make adjustments to your image; changes are automatically saved without affecting the original data.

Tap the image info icon to add a custom name and notes about your experiment. Tap the Send/Save icon to select your file format and destination, such as USB drive or network, and export the image.