Setting Up and Running Mini-PROTEAN® TGX™ Precast Gels

Remove the TGX gel from the foil and plastic-lined pouch by tearing at the notch. Peel off the tape from the bottom of the cassette gently but firmly.

Open the wing clamps on the electrode assembly module, load the TGX gels with the short plate facing inside, then close each wing clamp firmly to secure them.

Position the electrode assembly module into the Tetra cell tank, aligning the banana plugs with the tabs and ensuring the red anode marking lines up with the red hatch mark on the tank. Add the companion module if running three or four gels.

Grasp the comb from the center notch and lift straight up and out. Fill the inner chambers first, then the outer chamber with running buffer to the appropriate mark (2 or 4 gels). Rinse the wells with a transfer pipet.

Load protein samples into the wells. Place the lid on the Tetra tank, matching the colored electrodes on the lid to the electrode posts. Start the run and monitor until the sample dye front reaches the reference marker at the cassette bottom.

Turn off the power supply and remove the leads. Remove the Tetra cell lid and pour out the buffer. Open each wing clamp and remove the gels from the core assembly.

Use the opening lever supplied with the gel box, inserting the arrow between the cassette plates at each of the four marked points. Wedge the teeth of the lever between the plates and crack them open with upward or downward motion at all four points.

Peel the cassette plates apart. Remove the gel by inverting it over a buffer-filled tray to let it peel from the plate, or pick it up from the bottom and place it directly into staining solution.